Instruments
Andor Dragonfly spinning disk confocal microscope
- Nikon Ti-Eclipse epifluorescent microscope base
- ASI XYZ stage with Piezo Z-axis
- Two Andor Sona sCMOS cameras
- Mosaic FRAPPA (fluorescence recovery after photobleaching/photoactivation) system
- Micropoint laser ablation setup with 337nm tunable laser
- Multi-color TIRF capabilities
- OKOLAB stagetop incubator with temperature, humidity, and CO2 control
Objectives on Dragonfly Spinning Disk Confocal Microscope: | ||||
Magnification | Type | Numerical Aperature (NA) | Working Distance (WD, mm) | Immersion Media |
10x | Plan APO | 0.45 | 4.0 | Air |
20x | Plan Fluor | 0.75 | 0.33-0.51 | Oil, water, glycerol, silicone oil |
40x | LWD CFI APO with correction collar | 1.15 | 0.59-0.61 | Water |
60x | Plan APO with correction collar | 1.2 | 0.28-0.31 | Water |
60x | CFI APO TIRF with correction collar | 1.49 | 0.07-0.13 | Oil |
Excitation lasers (nm) | |||
405 | 488 | 561 | 637 |
Emission filter options (nm) | |||
DAPI 425-475 | GFP/FITC 500-550 | RFP/TRITC 575-625 | Cy5 705-745 |
Nikon Epifluorescence microscope
- Nikon Ti-Eclipse 2 epifluorescent microscope base with perfect focus
- ASI XYZ stage with Piezo Z-axis
- OKOLAB stagetop incubator with temperature, humidity, and CO2 control
- Andor Zyla sCMOS camera
Objectives on HCIC Nikon Epifluorescence Microscope: | ||||
Magnification | Type | Numerical Aperature (NA) | Working Distance (WD, mm) | Immersion Media |
4x | CFI Plan Fluor DL | 0.13 | 16.5 | Air |
10x | CFI Plan Fluor DL | 0.30 | 15.2 | Air |
20x | ELWD Plan Fluor with correction collar | 0.45 | 6.9-8.2 | Air |
20x | LWD Ph1 ADL | 0.40 | 3.0 | Air |
40x | ELWD Plan Fluor with correction collar | 0.60 | 2.8-3.6 | Air |
40x | LWD Ph1 ADL | 0.55 | 2.1 | Air |
Excitation wavelength options (CoolLED pE-4000, nm) | |||
365 | 460 | 525 | 635 |
385 | 470 | 560 | 660 |
405 | 490 | 580 | 740 |
435 | 500 | 595 | 770 |
Emission filter options (nm) | |||
DAPI 435-485 | FITC 515-555 | TRITC 577-630 | Cy5 663-738 |
LUMICKS C-Trap Dymo optical tweezers system
- 532 and 638nm fluorescence
- 3D-nanostage
- 2 optical traps and 2 force detectors
- Automated 5-channel microfluidics system
Etaluma Lumascope LS850
- Incubator-based microscope for long-term imaging
- Consistent 37℃ and 5% CO2 environment with HERAcell VIOS 160i CO2 incubator
- High QE Monochrome CMOS BSI Sensor, 4.4 Megapixel, 12-bit
- Automated XY stage with 1-3 micron positional reproducibility
- Z-stacks with 14mm range, 100nm step size, image-based autofocus
Objectives on HCIC Etaluma LS850: | ||||
Magnification | Type | Numerical Aperature (NA) | Working Distance (WD, mm) | Immersion Media |
2.5x | Plan Semi Apochromat | 0.07 | 5.7 | Air |
4x | U Plan Fluorite | 0.13 | 17 | Air |
10x | Achromat Phase IPC | 0.25 | 8.8 | Air |
20x | LWD Achromat Phase IPC | 0.4 | 3.2 | Air |
20x | LWD U Plan FL Objective with correction collar | 0.45 | 6.4-7.8 | Air |
40x | LWD Achromat Phase IPC | 0.55 | 2.2 | Air |
40x | LWD U Plan FL Objective with correction collar | 0.6 | 2.7-4.0 | Air |
60x | Plan Achromat Objective | 0.8 | 0.23 | Air |
Fluorescence Imaging Options | ||
Color | Excitation (nm) | Emission (nm) |
Blue | 370-410 | 429-462 |
Green | 473-491 | 502-561 |
Red | 580-598 | 612-680 |
Transmitted Light Imaging Options | ||
Brightfield | Auraphase phase contrast | |
FIRE custom lightsheet microscope
- Custom-built Single Objective Lightsheet (SOLS) microscope
- Thorlabs Fast XY-scanning stage and Z-axis piezo stage with 25nm resolution
- Andor Zyla sCMOS camera
- 535 μm x 535 μm usable field of view
Objectives on HCIC FIRE Lightsheet: | ||||
Magnification | Type | Numerical Aperature (NA) | Working Distance (WD, mm) | Immersion Media |
30x | UPlan Super Apochromat | 1.05 | 0.8 | Silicone Oil |
Excitation wavelengths (nm) | |||
405 | 488 | 561 | 638 |
Emission filter options (approx. nm) | |||
DAPI 415-470 | FITC 500-545 | TRITC 575-615 | Cy5 660-750 |
Zeiss LSM980 with Airyscan 2 (coming Summer 2024)
- 1 Megapixel CMOS Axiocam 807 monochrome camera
- 8 detectors: 1x Airyscan 2, 4x GaAsP, 2x PMT, 1x Transmitted PMT
- 5 lasers, 7 channel widefield illumination
- AI sample finder
Objectives on HCIC Zeiss LSM980: | ||||
Magnification | Type | Numerical Aperature (NA) | Working Distance (WD, mm) | Immersion Media |
5x | Plan Neofluar | 0.16 | 18.5 | Air |
10x | Plan-Neofluar | 0.3 | 5.2 | Air |
20x | Plan Apochromat | 0.8 | 0.55 | Air |
40x | Plan Apochromat with correction collar | 1.2 | 0.39-0.43 | Water, Oil, Silicone Oil, Glycerine |
63x | Plan Apochromat | 1.4 | 0.19 | Oil |
Excitation wavelengths (nm) | ||
Laser (confocal) | LED (widefield) | Example Fluorophores |
405 | 385 | DAPI, Alexa 405, Hoechst 33258 |
| 430 | CFP, Lucifer Yellow, Alexa 430 |
488 | 475 | GFP, FITC |
561 | 555 | Cy3, tritC, DsRed |
594 | 590 | mCherry, Alexa 568, mPlum |
639 | 630 | Cy5, Alexa 631 |
| 735 | Cy7 |
Image Analysis Workstations
- Imaris Essentials license
- Nikon Batch Deconvolution software
- FIJI/ImageJ
